The BMP-2/Smads signaling pathway promote the osteogenic differentiation of mesenchymal stem cells in rat with bone metabolism imbalance

doi:10.3877/cma.j.issn.2096-0263.2021.02.001

Abstract

Abstract:

Objective

The purpose of this study was to investigate the effect of BMP-2/Smads signaling pathway on osteogenic differentiation of bone marrow mesenchymal stem cells (rBMSCs) in rat with bone metabolism imbalance.

Methods

Ovaries of female SD rats were excised and OP model was established. The osteogenic differentiation of rBMSCs was identified by osteogenic induction. Fat induction and flow cytometry (FCM) were used to detect fat differentiation and the expression of rBMSC surface markers. rBMSCs were divided into blank group, negative control (NC) group (transfected with NC BMP-2 plasmid), SI-BMP2 group (transfected with low expressing BMP-2 plasmid) and OE-BMP2 group (transfected with high expressing BMP-2 plasmid). Western blot and quantitative real-time PCR were used to detect the expression of key factors and osteogenic related factors. The formation of calcified nodules was observed by alizarin red staining. ALP activity was determined by alkaline phosphatase staining.

Results

In OP group, the weight and bone mineral density of rats increased (P<0.01). rBMSCs of OP rats have the ability of osteogenic differentiation and lipogenic differentiation. CD44 (91.3±2.9%) and CD105 (94.8±2.1%) are highly expressed. Compared with the blank group, the level of BMP-2 and Smad1 in OE-BMP2 group increased, and the area of calcified nodule and ALP positive staining increased (P<0.05). The expression level of Runx2, OC and OPN in OE-BMP2 group was higher than that in blank group (P<0.05). The results of SI-BMP2 group were opposite to those of OE-BMP2 group.

Conclusion

The BMP signaling pathway activated by BMP-2 can promote the osteogenic differentiation of rBMSCs in OP rats.

Key words: BMP signaling pathway, Bone marrow mesenchymal stem cells, Osteoporosis, Osteogenic differentiation

Weili Zhang, Min Luo, Jiang Peng, Bin Zhao. The BMP-2/Smads signaling pathway promote the osteogenic differentiation of mesenchymal stem cells in rat with bone metabolism imbalance[J]. Chinese Journal of Geriatric Orthopaedics and Rehabilitation(Electronic Edition), 2021, 07(02): 65-72.

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