The difference of anti-osteoporosis effect of Puerarin on different parts of bone in postmenopausal osteoporosis rats

doi:10.3877/cma.j.issn.2096-0263.2023.01.005

Abstract

Abstract:

Objective

This study aims to investigate the effect of Puerarin on bone mineral density in rats through animal experiments, and explore the diversity of anti-osteoporosis effect of puerarin in different parts of bones.

Methods

Bilateral ovariectomy was performed on 24 female SD rats to establish the postmenopausal osteoporosis model. After the rats were treated with puerarin, the changes of bone mineral density (BMD) were detected by dual energy X-ray absorption (DXA) in the 2nd, 4th, 8th and 12th weeks. The differences of BMD and bone mineral content (BMC) in different parts were compared. The effect of Puerarin on the microstructure of bone tissue was evaluated by observing tissue sections.

Results

The effect of Puerarin was the most significant in the 8th week since it was applied. After Puerarin Treatment, the BMD and BMC of cancellous bone such as distal femur and lumbar spine increased significantly, and bone trabeculae of treatment group were thicker and more in quantity, while there was little change in cortical bone such as middle femur.

Conclusion

Puerarin has a stronger anti-osteoporosis effect on cancellous bone than cortical bone in postmenopausal osteoporosis rats.

Key words: Puerarin, Postmenopausal osteoporosis, Bone mineral density

Chengyu Qin, Haonan Zhou, Yuanming Chen. The difference of anti-osteoporosis effect of Puerarin on different parts of bone in postmenopausal osteoporosis rats[J]. Chinese Journal of Geriatric Orthopaedics and Rehabilitation(Electronic Edition), 2023, 09(01): 23-27.

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References 21

1	夏维波,章振林,林华,等.原发性骨质疏松症诊疗指南(2017) [J].中国骨质疏松杂志, 2019, 25(03): 281-309.
2	Rozenberg S, Al-Daghri N, Aubertin-Leheudre M, et al. Is there a role for menopausal hormone therapy in the management of postmenopausal osteoporosis? [J]. Osteoporosis International, 2020, 31(12): 2271-2286.
3	Wang S, Zhang S, Wang S, et al. A comprehensive review on Pueraria: Insights on its chemistry and medicinal value [J]. Biomed Pharmacother, 2020, 131: 110734.
4	Yang X, Zheng H, Liu Y, et al. Puerarin for OVX-Induced postmenopausal osteoporosis in murine model:systematic review and Meta-Analysis [J]. Curr Stem Cell Res Ther, 2020, 15(1): 37-42.
5	吕遐,扶琼.原发性骨质疏松症的研究进展与最新指南解读[J].临床内科杂志, 2020, 37(05): 319-322.
6	Marcus R. Post-menopausal osteoporosis [J]. Best Pract Res Clin Obstet Gynaecol, 2002, 16(3): 309-327.
7	Langdahl B, Ferrari S, Dempster DW. Bone modeling and remodeling:potential as therapeutic targets for the treatment of osteoporosis [J]. Ther Adv Musculoskelet Dis, 2016, 8(6): 225-235.
8	Yang H, Bullock WA, Myhal A, et al. Cancellous bone May have a greater adaptive strain threshold than cortical bone [J]. JBMR Plus, 2021, 5(5): e10489.
9	Clynes MA, Harvey NC, Curtis EM, et al. The epidemiology of osteoporosis [J]. Br Med Bull, 2020, 133(1): 105-117.
10	Hamada T, Matsubara H, Hikichi T, et al. Rat model of an autologous cancellous bone graft [J]. Sci Rep, 2021, 11(1): 18001.
11	Yin X, Wang X, Hu X, et al. ERβ induces the differentiation of cultured osteoblasts by both Wnt/β-catenin signaling pathway and estrogen signaling pathways [J]. Exp Cell Res, 2015, 335(1): 107-114.
12	Liu G, Lu Y, Mai Z, et al. Suppressing MicroRNA-30b by Estrogen Promotes Osteogenesis in Bone Marrow Mesenchymal Stem Cells [J]. Stem cells international, 2019, 2019: 7547506.
13	Li J, PENG Y. Effect of puerarin on osteogenic differentiation of human periodontal ligament stem cells [J]. The Journal of international medical research, 2020, 48(4): 300060519851641.
14	刘永红,陈远明,周昊楠,等.葛根素诱导脂肪干细胞成骨分化的实验研究[J].中国骨质疏松杂志, 2020, 26(05): 631-635.
15	Tiyasatkulkovit W, Charoenphandhu N, Wongdee K, et al. Upregulation of osteoblastic differentiation marker mRNA expression in osteoblast-like UMR106 cells by puerarin and phytoestrogens from Pueraria mirifica [J]. Phytomedicine, 2012, 19(13): 1147-1155.
16	Yasuda H. Discovery of the RANKL/RANK/OPG system [J]. J Bone Miner Metab, 2021, 39(1): 2-11.
17	Xiao L, Zhong M, Huang Y, et al. Puerarin alleviates osteoporosis in the ovariectomy-induced mice by suppressing osteoclastogenesis via inhibition of TRAF6/ROS-dependent MAPK/NF-κB signaling pathways [J]. Aging, 2020, 12(21): 21706-21729.
18	Suthon S, Jaroenporn S, Charoenphandhu N, et al. Anti-osteoporotic effects of Pueraria candollei var. mirifica on bone mineral density and histomorphometry in estrogen-deficient rats [J]. J Nat Med, 2016, 70(2): 225-233.
19	Lamy O, Stoll D, Aubry-Rozier B, et al. Stopping denosumab [J]. Curr Osteoporos Rep, 2019, 17(1): 8-15.
20	Zhou YX, Zhang H, Peng C. Puerarin: a review of pharmacological effects [J]. Phytotherapy research: PTR, 2014, 28(7): 961-975.
21	Li B, Wang Y, Gong S, et al. Puerarin improves OVX-induced osteoporosis by regulating phospholipid metabolism and biosynthesis of unsaturated fatty acids based on serum metabolomics [J]. Phytomedicine, 2022, 102: 154198.

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