Bioinformatics analysis of CD8+ T cells, follicular helper T cells and plasma cells infiltration in intervertebral disc degeneration

doi:10.3877/cma.j.issn.2096-0263.2021.04.001

Abstract

Abstract:

Objective

To screen the key genes of intervertebral disc degeneration (IDD) tissues and non-degeneration tissues, and to further analyze the infiltration of immune cells.

Methods

Download the IDD expression profile chip dataset from the GEO database, and use R software to screen differentially expressed genes (DEGs) in degenerate and non-degenerate tissues, and then construct a protein-protein interaction network (PPI). Screen out the hub gene. Finally, for the first time, a scientific deconvolution algorithm (CIBERSORT) was used to analyze the infiltration of immune cells in IDD tissues to reveal the role of immune cell infiltration in IDD.

Results

A total of 166 DEGs and 10 hub genes were screened. Immune cell infiltration analysis found that monocytes in IDD tissues were positively correlated with T cells regulatory, and eosinophils were negatively correlated with monocytes and T cells regulatory; CD8⁺T cells and follicular helper T cells were less infiltrated in IDD. while plasma cells are more infiltrated in IDD tissues; dendritic cells resting, plasma cells, NK cells resting and T cells regulatory interact strongly with other immune cells. T cells follicular helper, Macrophages M2, and B cells na?ve assist weak interaction with other immune cells.

Conclusions

Our results reveal the hub gene in IDD and immune cell infiltration analysis shows that CD8⁺T cells, follicular helper T cells, and plasma cells are closely related to the development of IDD. To provide a new idea for further exploring the molecular mechanism of IDD.

Key words: Intervertebral disc degeneration, CD8⁺ T cells, Follicular helper T cells, Plasma cells, CIBERSORT

Yicheng Gao, Guangzhi Zhang, Qiqi Xie, Yajun Deng, Enhui Ren, Zuolong Wu, Xuegang He, Xuewen Kang. Bioinformatics analysis of CD8⁺ T cells, follicular helper T cells and plasma cells infiltration in intervertebral disc degeneration[J]. Chinese Journal of Geriatric Orthopaedics and Rehabilitation(Electronic Edition), 2021, 07(04): 193-200.

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排名	基因简写	基因全称	评分
1	TP53	Tumor Protein P53	15
2	SLC2A8	Solute Carrier Family 2 Member 8	5
2	BMP4	Bone Morphogenetic Protein 4	5
4	CALM1	Calmodulin 1	4
4	KLC4	Kinesin Light Chain 4	4
6	LTV1	LTV1 Ribosome Biogenesis Factor	3
6	WDR46	WD Repeat Domain 46	3
6	NDUFS4	NADH:Ubiquinone Oxidoreductase Subunit S4	3
6	AMPH	Amphiphysin	3
6	SH3GL2	SH3 Domain Containing GRB2 Like 2, Endophilin A1	3

排名	基因简写	基因全称	评分
1	TP53	Tumor Protein P53	15
2	SLC2A8	Solute Carrier Family 2 Member 8	5
2	BMP4	Bone Morphogenetic Protein 4	5
4	CALM1	Calmodulin 1	4
4	KLC4	Kinesin Light Chain 4	4
6	LTV1	LTV1 Ribosome Biogenesis Factor	3
6	WDR46	WD Repeat Domain 46	3
6	NDUFS4	NADH:Ubiquinone Oxidoreductase Subunit S4	3
6	AMPH	Amphiphysin	3
6	SH3GL2	SH3 Domain Containing GRB2 Like 2, Endophilin A1	3

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