MiR-3182 regulates LPPR4 expression and inhibits osteoblasts differentiation and maturation

doi:10.3877/cma.j.issn.2096-0263.2021.03.009

Abstract

Abstract:

Objective

This study aims to reveal the regulatory effect of miR-3182 and its target LPPR4 on osteoblasts differentiation and maturation.

Methods

The study employed GSE63446 and GSE62402 datasets to reveal the association of circulating miRNA and tissue mRNA in bone mineral density (BMD). The two datasets were cross validated based on targets prediction. Plasmid mediated LPPR4 gene overexpression or siRNA interference was used to investigate its regulatory role in osteoblasts differentiation and maturation.

Results

Circulating miR-3182 was highly expressed in low BMD group, indicating excellent sensitivity and specificity in BMD classification. Lentivirus plasmind mediated miR-3182 expression decreased LPPR4 protein level and inhibited osteoblast hFOB1.19 mineralized nodules formation. Ectogenic overexpression of LPPR4 promoted hFOB1.19 cells mineralized nodule formation, and induced biomarker OPG expression.

Conclusion

LPPR4 promotes osteoblasts hFOB1.19 differentiation and maturation.

Key words: MicroRNA-3182, LPPR4 protein, Bone density, Gene Expression Omnibus (GEO) Database

XiaoLin Tang, Xia Kong, Huaigao Wang, Rong Li. MiR-3182 regulates LPPR4 expression and inhibits osteoblasts differentiation and maturation[J]. Chinese Journal of Geriatric Orthopaedics and Rehabilitation(Electronic Edition), 2021, 07(03): 176-180.

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Figures/Tables 5

References 14

1	Wainwright SA, Marshall LM, Ensrud K, et al. Study of osteoporotic fractures research G:hip fracture in women without osteoporosis [J]. J Clin Endocrinol Metab, 2005, 90(5): 2787-2793.
2	Kim NH, Hyun SK, Li XY, et al. A p53/miRNA-34 axis regulates Snail1-dependent cancer cell epithelial-mesenchymal transition [J]. J Exp Med, 2011, 208(12): i32-i32.
3	Hayes J, Peruzzi PP, Lawler S. MicroRNAs in cancer: biomarkers, functions and therapy [J]. Trends Mol Med, 2014, 20(8): 460-469.
4	Tang J, Li Y, Liu K, et al. Exosomal miR-9-3p suppresses HBGF-5 expression and is a functional biomarker in hepatocellular carcinoma [J]. Minerva medica, 2018, 109(1): 15-23.
5	Pethő A, Chen YH, George A. Exosomes in extracellular matrix bone biology [J]. Curr Osteoporos Rep, 2018, 16(1): 58-64.
6	Upala S, Yong WC, Sanguankeo A. Bone mineral density is decreased in fibromyalgia syndrome: a systematic review and meta-analysis [J]. Rheumatol Int, 2017, 37(4): 617-622.
7	Tang XL, Lin JT, Wang GH, et al. MicroRNA-433-3p promotes osteoblast differentiation through targeting DKK1 expression [J]. PLoS One, 2017, 12(6): e0179860.
8	周琨，陆霁，殷晓星，等. 血清miRNA-128a和miRNA-128b表达对胃癌早期诊断和预后的影响[J]. 复旦学报(医学版)，2019，06：15-19.
9	许从飞，王均. 非编码RNA药物的研究进展[J]. 生命科学，2018，30(2)：213-221.
10	Karabegovic I, Maas S, Medina-Gomez C, et al. Genetic Polymorphism of miR-196a-2 is Associated with Bone Mineral Density (BMD) [J]. Int J Mol Sci, 2017, 18(12): 2529.
11	Kelch S, Elizabeth RB, Seeliger C, et al. miRNAs in bone tissue correlate to bone mineral density and circulating miRNAs are gender Independent in osteoporotic patients [J]. Sci Rep, 2017, 7(1): 15861.
12	Zang D, Zhang C, Li C, et al. LPPR4 promotes peritoneal metastasis via Sp1/integrin alpha/FAK signaling in gastric cancer [J]. Am J Cancer Res, 2020, 10(3): 1026-1044.
13	Yu P, Agbaegbu C, Malide DA, et al. Cooperative interactions of LPPR family members in membrane localization and alteration of cellular morphology [J]. J Cell Sci, 2015, 128(17): 3210-3222.
14	章里西. microRNA对冠心病疑似致病基因LPPR4表达调控的初步研究[D]. 北京：北京协和医学院，2017.

序号	KEGG通路	p-value	基因名称
1	Prion diseases (hsa05020)	1.81612E-76	PRNP
2	RNA transport (hsa03013)	0.04291703	SENP2，NUP43
3	Systemic lupuserythematosus (hsa05322)	0.04291703	C2

序号	KEGG通路	p-value	基因名称
1	Prion diseases (hsa05020)	1.81612E-76	PRNP
2	RNA transport (hsa03013)	0.04291703	SENP2，NUP43
3	Systemic lupuserythematosus (hsa05322)	0.04291703	C2

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