Effect of 3D printed collagen / hydroxyapatite scaffold on osteogenic differentiation of bone marrow mesenchymal stem cells

doi:10.3877/cma.j.issn.2096-0263.2020.03.001

Abstract

Abstract:

Objective

To investigate the effect of 3D printed collagen/ hydroxyapatite scaffold on osteogenic differentiation of bone marrow mesenchymal stem cells.

Methods

Rat BMSCs were isolated from rat bone marrow. Cell proliferation and differentiation were detected by MTT and ALP activity. After inducing cell culture for 17 days, cells in each group were stained with alizarin red to observe the staining of calcium nodules.

Results

The MTT results showed that there was no significant difference in OD between the extraction group and the control group at different time point (P>0.05). ALP activity results showed that the extraction group had significant differences compared with the control group at different time point (P<0.05); the extraction induction group had significant differences at different time point compared with the extraction group (P<0.05); At 48 h and 72 h, the group had significant differences compared with the extraction group (P<0.05). Alizarin red staining showed that the cells in the control group had no calcium knot nodes. The red area staining was clearly observed under the microscope in the extraction group and the induction group, and the calcium knot nodes were observed under the microscope. The number, size and color depth of the knot nodes were significantly better than those of the other groups.

Conclusion

The scaffold has good biocompatibility, can promote the differentiation of BMSCs to osteogenesis, and has low cytotoxicity. It is suitable for the treatment of bone defect.

Key words: Collagen, Hydroxyapatite, Mesenchymal Stem Cells, Osteogenic differentiation

Bo Wu, Ye Liu, Xu Ma, Chunsheng Zhi, Mingchang Du, Liangquan Zhai, Zhengbo Yang, Jiayuan Wang, Yihan Wang. Effect of 3D printed collagen / hydroxyapatite scaffold on osteogenic differentiation of bone marrow mesenchymal stem cells[J]. Chinese Journal of Geriatric Orthopaedics and Rehabilitation(Electronic Edition), 2020, 06(03): 123-127.

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References 25

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组别	24 h	48 h	72 h	96 h	F值	P值
对照组	0.681±0.017	0.942±0.034	1.436±0.043	1.514±0.039	1060.99	<0.01
浸提组	0.676±0.018	0. 921±0.028	1.428±0.035	1.506±0.041	1244.957	<0.01
F值	0.332	1.670	0.223	0.121
P值	0.574	0.217	0.644	0.733

组别	24 h	48 h	72 h	96 h	F值	P值
对照组	0.681±0.017	0.942±0.034	1.436±0.043	1.514±0.039	1060.99	<0.01
浸提组	0.676±0.018	0. 921±0.028	1.428±0.035	1.506±0.041	1244.957	<0.01
F值	0.332	1.670	0.223	0.121
P值	0.574	0.217	0.644	0.733

组别	24 h	48 h	72 h	96 h	F值	P值
对照组	10.42±0.36	12. 54±0.58	12.13±0.61	11.73±0.54	23.685	<0.01
浸提组	14.71±1.79^a	18.38±1.83^a	17.25±1.63^a	15.912±1.58^a	7.035	0.001
诱导组	16.42±1.62^a	23.16±1.93^ab	20.85±1.95^ab	17.96±2.04^a	20.133	<0.01
浸提诱导组	17.37±2.06^ab	25.83±2.12^ab	20.44±1.86^ab	18.46±2.06^a	28.085	<0.01
F值	31.537	92.369	52.753	26.818
P值	<0.01	<0.01	<0.01	<0.01

组别	24 h	48 h	72 h	96 h	F值	P值
对照组	10.42±0.36	12. 54±0.58	12.13±0.61	11.73±0.54	23.685	<0.01
浸提组	14.71±1.79^a	18.38±1.83^a	17.25±1.63^a	15.912±1.58^a	7.035	0.001
诱导组	16.42±1.62^a	23.16±1.93^ab	20.85±1.95^ab	17.96±2.04^a	20.133	<0.01
浸提诱导组	17.37±2.06^ab	25.83±2.12^ab	20.44±1.86^ab	18.46±2.06^a	28.085	<0.01
F值	31.537	92.369	52.753	26.818
P值	<0.01	<0.01	<0.01	<0.01

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