Reconstruct large osteochondral defects of weight-bearing area by hIGF-I gene enhanced Mosaicplasty technique

doi:10.3877/cma.j.issn.2096-0263.2019.05.009

Abstract

Abstract:

Objective

To investigate the outcome of hIGF-I gene enhanced Mosaicplasty to repair large-sized osteochondral compound defects.

Methods

Osteochondral defects were created on the femoral condyle with the customed Mosaicplasty instruments in a diameter of 6 mm. Osteochondral plugs were harvested around the intercondylar fossa or intertrochlea groove, and pressed into the recipient sites by special instruments in a mosaic mode. hIGF-I gene enhanced tissue engineering was then applied to fill the "dead space" after Mosaicplasty. From 4 to 16 weeks postoperatively, the animals were sacrificed and the specimens were investigated in gross and under electromicroscopy as well as MRI detection.

Results

The transplanted subchondral bone and superficial cartilage was integrated hardly with each other or with recipient sites in hIGF-I enhanced Mosaicplasty groups. The quality and appearance of the transplanted and regenerated cartilage was similar to normal hyaline cartilage. Under microscopy, the regenerated cartilage was integrated with neighbor tightly in regular arrange. ECM distributed evenly and deeply stained by alcian blue. MRI analysis also demonstrated the healing process between the subchondral bone other than the contrast groups. The effectiveness of the GETE modified Mosaicplasty groups was apparently better than BMSCs modified Mosaicplasty groups and contrast groups.

Conclusions

hIGF-I gene enhanced tissue engineering can ameliorate the outcome of Mosaicplasty to repair the osteochondral defects in large size.

Key words: Insulin-like growth factor, Genes, Osteochondral, Mosaicplasty

Jie Zhang, Hui Huang, cuiping Jiang, Yi Sun, Xiaofei Li, Haining Zhang, Yingzhen Wang. Reconstruct large osteochondral defects of weight-bearing area by hIGF-I gene enhanced Mosaicplasty technique[J]. Chinese Journal of Geriatric Orthopaedics and Rehabilitation(Electronic Edition), 2019, 05(05): 291-296.

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References 16

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