Surface fucosylation of human adipose-derived stem cells (ASCs) augments homing to bone

doi:10.3877/cma.j.issn.2096-0263.2018.05.007

Abstract

Abstract:

Objective

To investigate whether the expression of CD44 on adipose-derived stem cells can be modified by glycosylation technology to become hematopoietic stem cell E/L selectin ligand (HCELL), which effectively enhance bone marrow homing and differentiate into bone.

Methods

α-1,3-fucosyltransferase (e.g., fucosyltransferase V (FTV) and its substrate GDP-fucose were used to treat human adipose-derived stem cells, so that the cell surface CD44 molecule glycosylated and turned into HCELL, and its cell biology characteristics such as proliferation and differentiation were detected. Parallel plate flow chamber test and immunofluorescence staining confirmed the homing and osteogenic ability of infusion cellls.

Results

α-1,3-fucosylated hASCs express HCELL without impairing cell viability can induce E-selectin binding on vascular endothelial cells, and make hASCs and endothelials under shear conditions. E-selectin produces a powerful rolling adhesion effect that promotes the migration of hASCs into the bone marrow and produces human osteoblast-lineage cells in the bone marrow of mice.

Conclusion

This study demonstrates that glycosylation technology can enhance the bone marrow homing ability of stem cells, and does not have a negative effect on stem cell viability.

Key words: Adipose tissue-derived stem cells, Homing, Bone regeneration, Fucosylation

Xin Jin, Baoquan Wang, Wei Tong, Xianzhe Liu, Xiaoguang Zhang, Jiarui Fang, Song Li, Yuqiong Zhang, Rabi M Dhakal, Jian Wang, Shuhua Yang, Hongtao Tian. Surface fucosylation of human adipose-derived stem cells (ASCs) augments homing to bone[J]. Chinese Journal of Geriatric Orthopaedics and Rehabilitation(Electronic Edition), 2018, 04(05): 287-295.

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References 32

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